cleaved n terminal gsdmd antibody  (MedChemExpress)

Journal: mSystems

Article Title: Taurine attenuates Listeria monocytogenes -induced inflammation and pyroptosis in mouse model by regulating MAPK and NLRP3/caspase-1/GSDMD pathways

doi: 10.1128/msystems.01043-25

Figure Lengend Snippet: Proposed mechanism of taurine against Lm infection. Taurine targets Lm virulence and host pyroptosis. Taurine inhibits bacterial pathogenesis while balancing immune responses: activating MAPK signaling pathway for T-cell immunity and suppressing NLRP3/GSDMD-mediated pyroptosis.

Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE gel electrophoresis and transferred to PVDF membrane with 5% skimmed milk closed for 1 h. The target proteins on the membranes were probed with the specific primary antibodies as follows: rabbit anti-JNK1 + JNK2 + JNK3 polyclonal antibody (Bioss, bs-2592R), rabbit anti-phospho-JNK1/2/3 (T183 + T183 + T221) monoclonal antibody (Bioss, bsm-52462R), rabbit anti-ERK1/2 monoclonal antibody (Bioss, bsm-52259R), rabbit anti-phospho-ERK1/2 (Thr202 + Thr204) polyclonal antibody (Bioss, bs-3016R), p38 MAPK rabbit mAb (CST, 8690), phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb (CST, 4511), IL-1β pAb (Bioss, bs-812R), caspase 1/p20/p10 polyclonal antibody (Proteintech, 22915-1-AP), total and cleaved N-terminal GSDMD antibody (Abmart, P79887R), and NLRP3 monoclonal antibody (Proteintech, 68102-1-Ig).

Techniques: Infection

Journal: BMC Immunology

Article Title: Diagnostic and prognostic value of deregulated miR-6822-3p in patients with severe pneumonia

doi: 10.1186/s12865-026-00801-3

Figure Lengend Snippet: miR-6822-3p promotes pyroptosis in THP-1 cells. A Expression levels of miR-6822-3p in THP-1 cells. B Western blot (WB) analysis of pyroptosis-related proteins in THP-1 cells. C The quantification of NLRP3 by WB. D The quantification of cleaved caspase-1 by WB. E The quantification of cleaved GSDMD by WB. F IL-1β levels in THP-1 cell supernatants. G IL-18 levels in THP-1 cell supernatants. H Changes in VE-cadherin mRNA expression in HLMVEC cells

Article Snippet: The primary antibodies used in the experiment were NLRP3 (Beyotime, AF2155), cleaved caspase-1 (MCE, HY- P80622 ), cleaved N-terminal GSDMD (MCE, HY- P86158 ), and β-actin (Beyotime, AF5003).

Techniques: Expressing, Western Blot

Journal: BMC Anesthesiology

Article Title: Esketamine mitigates endotoxin-induced acute lung injury by suppressing caspase-11-driven pyroptosis

doi: 10.1186/s12871-025-03220-w

Figure Lengend Snippet: A and C : The expression of caspase-11 protein in each group was detected by immunohistochemistry, CLP vs. Sham ( P < 0.0001), CLP + ES vs. CLP ( P < 0.0001). B and D : The expression of GSDMD-N protein in each group was detected by immunohistochemistry, CLP vs. Sham ( P < 0.0001), CLP + ES vs. CLP ( P < 0.0001). *** P < 0.001, **** P < 0.0001

Article Snippet: All drugs, commercial antibodies and reagents were purchased from the following sources: esketamine (230619BL, Jiangsu Hengrui Pharmaceuticals, Jiangsu, China), rabbit Two-Step Kit (PV-6001, ZSGB-BIO, Beijing, China), DAB chromogenic kit (ZLI-9018, ZSGB-BIO, Beijing, China), rabbit polyclonal antibody to Caspase-11 (DF7609, Affinity Biosciences LTD, Jiangsu, China, 1:1000), Cleaved GSDMD (N Terminal) Rabbit mAb (A22523, ABclonal Technology, Wuhan, China, 1:500), GAPDH Recombinant Rabbit Monoclonal Antibody (ET1601-4, HUABIO, Hangzhou, China,1:5000), Phospho-PI3-kinase p85-alpha/gamma Tyr467/199 ( T40116 , Abmart, Shanghai, China, 1:1000), Phospho-AKT1/2/3 Ser473 (AF0016, Affinity Biosciences LTD, Jiangsu, China, 1:1000), WB ultra-high-efficiency RIPA lysis buffer (PR21001-S, zsgentech, Tianjin, China), protease inhibitor cocktail (RW0102, Report BIOTECH, Hebei, China), phosphatase inhibitor cocktail (RW0103, Report BIOTECH, Hebei, China), BCA Protein Assay Kit (P1511, APPLYGEN, Beijing, China), protein loading buffer solution (WA0301, Report BIOTECH, Hebei, China), three-color protein Marker (PR24001, zsgentech, Tianjin, China), FuturePAGETM 12% 12 Wells (ET12012Gel, ACE biotechnology, Changzhou, China), PVDF membrane (IPVH00010-10, zsgentech, Tianjin, China), goat anti-rabbit IgG H + L, HRP (S1002-100, Report BIOTECH, Hebei, China), super ECL Plus Ultrasensitive Luminescent Solution (P1050-100, APPLYGEN, Beijing, China), TRI RNA Lysis Solution ( M11008 , zsgentech, Tianjin, China), 6 min 1 st Strand cDNA Synthesis Kit ( M14003 , zsgentech, Tianjin, China), 2×ZAPA3G SYBR Green qPCR Mix ( M13002 , zsgentech, Tianjin, China), Rat Interleukin 1 Beta (IL-1β) ELISA Kit (JL20884, JONLNBIO, Shanghai, China).

Techniques: Expressing, Immunohistochemistry

Journal: BMC Anesthesiology

Article Title: Esketamine mitigates endotoxin-induced acute lung injury by suppressing caspase-11-driven pyroptosis

doi: 10.1186/s12871-025-03220-w

Figure Lengend Snippet: A : The expression of caspase-11 and GSDMD-N protein in each group was detected by WB, caspase-11: CLP vs. Sham ( P < 0.01), CLP + ES vs. CLP ( P < 0.05), GSDMD-N: CLP vs. Sham ( P < 0.01), CLP + ES vs. CLP ( P < 0.05). B : The expression of caspase-11 and GSDMD mRNA in each group was detected by qrt-PCR, caspase-11: CLP vs. Sham ( P < 0.01), CLP + ES vs. CLP ( P < 0.01), GSDMD: CLP vs. Sham ( P < 0.0001), CLP + ES vs. CLP ( P < 0.001). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: All drugs, commercial antibodies and reagents were purchased from the following sources: esketamine (230619BL, Jiangsu Hengrui Pharmaceuticals, Jiangsu, China), rabbit Two-Step Kit (PV-6001, ZSGB-BIO, Beijing, China), DAB chromogenic kit (ZLI-9018, ZSGB-BIO, Beijing, China), rabbit polyclonal antibody to Caspase-11 (DF7609, Affinity Biosciences LTD, Jiangsu, China, 1:1000), Cleaved GSDMD (N Terminal) Rabbit mAb (A22523, ABclonal Technology, Wuhan, China, 1:500), GAPDH Recombinant Rabbit Monoclonal Antibody (ET1601-4, HUABIO, Hangzhou, China,1:5000), Phospho-PI3-kinase p85-alpha/gamma Tyr467/199 ( T40116 , Abmart, Shanghai, China, 1:1000), Phospho-AKT1/2/3 Ser473 (AF0016, Affinity Biosciences LTD, Jiangsu, China, 1:1000), WB ultra-high-efficiency RIPA lysis buffer (PR21001-S, zsgentech, Tianjin, China), protease inhibitor cocktail (RW0102, Report BIOTECH, Hebei, China), phosphatase inhibitor cocktail (RW0103, Report BIOTECH, Hebei, China), BCA Protein Assay Kit (P1511, APPLYGEN, Beijing, China), protein loading buffer solution (WA0301, Report BIOTECH, Hebei, China), three-color protein Marker (PR24001, zsgentech, Tianjin, China), FuturePAGETM 12% 12 Wells (ET12012Gel, ACE biotechnology, Changzhou, China), PVDF membrane (IPVH00010-10, zsgentech, Tianjin, China), goat anti-rabbit IgG H + L, HRP (S1002-100, Report BIOTECH, Hebei, China), super ECL Plus Ultrasensitive Luminescent Solution (P1050-100, APPLYGEN, Beijing, China), TRI RNA Lysis Solution ( M11008 , zsgentech, Tianjin, China), 6 min 1 st Strand cDNA Synthesis Kit ( M14003 , zsgentech, Tianjin, China), 2×ZAPA3G SYBR Green qPCR Mix ( M13002 , zsgentech, Tianjin, China), Rat Interleukin 1 Beta (IL-1β) ELISA Kit (JL20884, JONLNBIO, Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: Journal of orthopaedic surgery and research

Article Title: Exploring the mechanism by which UCHL3 alleviates diabetic foot ulcers: FOXM1/NLRP3 inflammasome-mediated angiogenesis and endothelial cell pyroptosis.

doi: 10.1186/s13018-025-05914-w

Figure Lengend Snippet: Fig. 5 Knockdown of FOXM1 leads to pyroptosis of endothelial cells and inhibits angiogenesis. (A) WB detection of FOXM1, NLRP3, and N-GSDMD expres sion in HUVECs. (B) CCK-8 assay measuring proliferation activity of HUVECs. (C) Scratch wound healing assay detecting migration ability of HUVECs. (D) Tube formation assay evaluating angiogenesis capacity of HUVECs. n = 3. The magnification of images D and E is 100 times (scale = 400 μm). *** P < 0.001, **** P < 0.0001. Three or more sets of data will be analyzed using one-way or two-way ANOVA, and Tukey’s will be used for post hoc testing

Article Snippet: Anti-TSP-1 antibody (1:2000, HY-P83750, MedChemExpress, New Jersey, USA), Anti-UCHL3 antibody (1:1000, A0280, Abclonal, Düsseldorf, Germany), Anti-UCHL3 antibody (1:10,000, ab126621, Abcam, Cambridge, Massachusetts, USA), anti-FOXM1 antibody (1:1000, ab180710, Abcam), anti-NLRP3 antibody (1:1000, 30109-1-AP, Santa Cruz Biotechnology, Inc., Shanghai, China), anti-cleaved N-terminal GSDMD antibody (1:1000, ab215203), UB (1:60,000, 80992-1-RR, Proteintech Group, Wuhan, China), and anti-GAPDH antibody (1:5000, 4A9L6, Thermo Fisher Scientific) were added to the membranes and incubated overnight at 4 °C.

Techniques: Knockdown, CCK-8 Assay, Activity Assay, Wound Healing Assay, Migration, Tube Formation Assay